這方法已自1977年開始試驗但很少公開。
Koger 1977, "Calcium Chloride, Practical Necrotizing Agent", Journal of the American Association of Bovine Practitioners (USA), (Nov 1977), v. 12, p. 118-119
可在美國PubMed資料庫上找尋研究學者Jana K 和Samanta PK。
"Clinical evaluation of non-surgical sterilization of male cats with single intra-testicular injection of calcium chloride".
http://www.ncbi.nlm.nih.gov/pubmed?term=(Jana%20K)%20AND%20Samanta%20PK%20AND%20calcium